PanWSGA enables researchers to project a single bacterial genome onto a curated, species-level pangenome reference derived from more than 100,000 high-quality assemblies.
The platform integrates standardized reannotation, a custom classification engine, and a dedicated visualization module to deliver reproducible gene-category profiles and functional summaries within minutes.
PanWSGA classifies genes from a single genome as core, accessory or unique by mapping them to a curated species-level pangenome. This provides pangenome-level context even when no multi-genome dataset is available.
A dedicated visualization subsystem that integrates classification and EggNOG functional annotations of Uniques genes to produce structured, publication-ready biological summaries. GeneEye supports both static high-resolution outputs and interactive browser-based
A dedicated visualization subsystem that integrates classification and EggNOG functional annotations to produce structured, publication-ready biological summaries. GeneEye supports both static high-resolution outputs and interactive browser-based charts.
PanWSGA includes 1,975 species-level pangenomes, each constructed from filtered, uniformly reannotated genomes and manually reviewed to remove cluster inconsistencies, paralog inflation, and contamination.
All analyses follow a fixed, standardized workflow-Prokka → Unique Gene Finder → EggNOG-mapper → GeneEye-executed in a containerized environment for consistency and reproducibility across submissions. (just bold the Unique Gene Finder & GeneEye) not others.
All computational steps run on the server. No installation, local dependencies, or computational infrastructure are required.
a genome assembly in FASTA format
The genome is reannotated using Prokka
Unique Gene Finder maps predicted proteins to pangenome clusters
EggNOG-mapper assigns COG/KEGG/EC annotations
GeneEye generates static and dynamic summaries
Users receive full output files, annotation tables, and publication-ready figures
Typical runtime: 2–20 minutes depending on genome size
PanWSGA serves diverse research and practical needs across multiple domains
Quickly place new bacterial isolates within species-level pangenome context, enabling immediate understanding of gene content relationships and evolutionary positioning.
Identify rare, unusual, or unique gene content that distinguishes your genome from the species pangenome, revealing potential virulence factors, resistance genes, or novel functions.
Compare functional annotations between core, accessory, and unique gene sets to understand metabolic capabilities and potential adaptations.
Generate high-quality figures, annotation tables, and summaries suitable for direct inclusion in research publications and presentations.
Characterize clinical isolates, identify virulence factors, and understand pathogen evolution
Track emerging strains, monitor antimicrobial resistance, and support public health initiatives
Support comparative genomics studies, evolutionary analyses, and functional genomics research
Teach pangenome concepts, bioinformatics workflows, and genomic analysis methods